Fig. 1
From: BZR1-NAC29-NAC31 signaling promotes the sheath blight resistance and tillering in rice
BZR1 interacts with NAC29 and NAC31. a, b Y2H assays showed the interaction between BZR1 and NAC29 or NAC31. The transformants were grown on SD-Leu-Trp (SD-LT) and SD-Leu-Trp-His (SD-LTH) plates containing 2 mM 3-AT, a histidine biosynthesis inhibitor. c BiFC assay showed the BZR1 interaction with NAC29 or NAC31 in the nucleus. Co-expression of BZR1-nYFP, NAC29-cCFP or NAC31-cCFP, and H2B-mRFP1 in N.benthamiana leaves. GFP is an indicator of protein–protein interactions. Scale bars = 20 μm. d CoIP assay showed BZR1 interaction with NAC29 or NAC31. Co-expression of BZR1-3 × FLAG and NAC29-GFP or NAC31-GFP in N.benthamiana leaves. Total proteins were extracted and immunoprecipitated with anti-FLAG beads, then subjected to western blot assay with anti-FLAG and anti-GFP antibodies