Fig. 5

Transcriptional profile analysis during S. bicolor infection by C. sublineola. a Comparison of sorghum anthracnose symptoms at 5 DPI using spray infection on sorghum cultivars BTx623 and GJH1. The former is disease-resistant, while the latter is susceptible. b Number of NLR genes with different expression levels in the two cultivars. Very high expression: FPKM > 10; High expression: 1 < FPKM < 10; Low expression: 0.1 < FPKM < 1; No expression: FPKM < 0.1. c Venn diagram comparing differentially responsive NLR genes to C. sublineola infection in BTx623 and GJH1 at 24 dpi and 48 dpi. d Temporal co-expression analysis revealing 14 modules of sorghum gene expression during C. sublineola infection. e Bioinformatic analysis of the transcriptional regulatory networks of NLR responses to early C. sublineola infection. Red squares represent NLRs, and green circles represent transcription factors (TFs). Green arrowheads indicate the key TFs