Fig. 2

Effect of π-stackings on CP accumulation. a GFP fluorescence and b relative GFP fluorescence intensities in the N. benthamiana leaf patches expressing GFP-TVBMVCP, one of its 16 mutants derived from aromatic residues (F⁹⁴, W⁹⁷, Y⁹⁸, Y¹⁰⁵, W¹²², W¹³⁶, Y¹⁴⁷, F¹⁶⁰, F¹⁶⁷, Y¹⁷⁴, Y¹⁸⁴, Y¹⁸⁸, Y²⁰², F²⁰⁴, F²⁰⁶ and Y²⁰⁷) that were predicted to form π-stacking, or one of its 3 mutants derived from aromatic residues (F⁴⁶, Y⁷⁷ and F²³⁷) that could not form π-stacking at 5 dpai. ‘Δ’ indicates the codon for the residue was deleted. The values are the means ± SD from three biological replicates per treatment. Treatments marked with the same lowercase letters have no significant statistical difference